This public tender process is for NGS Library Adapters (also "Adapters") and Hybridization Probes (also "Probes") used in the diagnostic whole exome sequencing (WES) NGS pipelines of HUSLAB, Laboratory of Genetics. The pipeline is used for preparing high quality sample libraries for Illumina NovaSeq 6000 sequencer. The HUSLAB WES workflow is based on a target enrichment step using hybridization probes to enrich DNA fragments originating from the genomic Regions of Interest ("RoI"). HUSLAB WES is used for high sensitivity variant detection of both germline and somatic DNA variations.
The Contracting Entity estimates that annual orders will be made for 2000 samples (estimation for 2022 and HUS estimates, that the number of samples is rising) divided in three to four (3-4) different separate orders.
The tender is devided into two lots:
1) NGS Library Adapters
2) Hybridization Probes
The procurement entity selects one supplier for both lots so that the lot may have either the same or a different supplier.
INTENDED USE
The Adapters and the Probes offered will be Components of the HUSLAB WES process. HUSLAB will select the Components and combine them in order to create the final HUSLAB WES process used for generation of high quality and high sensitivity customized WES sample libraries, fulfilling the Special Requirements for High Sensitivity Variant Detection.
The WES pipelines using the Adapters and the Probes are used for production of high quality WES sample libraries from DNA isolated from:
- whole blood
- freshly isolated or frozen tissue samples or cells
- FFPE samples (optionally omitting DNA fragmentation)
- cell-free DNA (omitting input DNA fragmentation)
The NGS pipelines using the Components allow for HUSLAB to build NGS pipelines fulfilling the accreditation requirements of SFS-EN ISO 15189:2012.
Please see paragraph II.2.4 for detailed description and Annex 1.1.1 for description of the steps of the current HUSLAB WES laboratory process and The Special Requirements for High Sensitivity Variant Detection. Annex 1.1.1 applies to both lots 1 and 2.
Määräaika
Tarjousten vastaanottamisen määräaika oli 2022-04-08.
Hankinta julkaistiin 2022-03-01.
Toimittajat
Seuraavat toimittajat mainitaan hankintapäätöksissä tai muissa hankinta-asiakirjoissa:
Kohde Hankinnan laajuus
Otsikko: Exome sequencing reagents - NGS library adapters and hybridization probes
Tuotteet/palvelut: Laboratorioreagenssit📦
Lyhyt kuvaus:
“This public tender process is for NGS Library Adapters (also "Adapters") and Hybridization Probes (also "Probes") used in the diagnostic whole exome...”
Lyhyt kuvaus
This public tender process is for NGS Library Adapters (also "Adapters") and Hybridization Probes (also "Probes") used in the diagnostic whole exome sequencing (WES) NGS pipelines of HUSLAB, Laboratory of Genetics. The pipeline is used for preparing high quality sample libraries for Illumina NovaSeq 6000 sequencer. The HUSLAB WES workflow is based on a target enrichment step using hybridization probes to enrich DNA fragments originating from the genomic Regions of Interest ("RoI"). HUSLAB WES is used for high sensitivity variant detection of both germline and somatic DNA variations.
The Contracting Entity estimates that annual orders will be made for 2000 samples (estimation for 2022 and HUS estimates, that the number of samples is rising) divided in three to four (3-4) different separate orders.
The tender is devided into two lots:
1) NGS Library Adapters
2) Hybridization Probes
The procurement entity selects one supplier for both lots so that the lot may have either the same or a different supplier.
INTENDED USE
The Adapters and the Probes offered will be Components of the HUSLAB WES process. HUSLAB will select the Components and combine them in order to create the final HUSLAB WES process used for generation of high quality and high sensitivity customized WES sample libraries, fulfilling the Special Requirements for High Sensitivity Variant Detection.
The WES pipelines using the Adapters and the Probes are used for production of high quality WES sample libraries from DNA isolated from:
- whole blood
- freshly isolated or frozen tissue samples or cells
- FFPE samples (optionally omitting DNA fragmentation)
- cell-free DNA (omitting input DNA fragmentation)
The NGS pipelines using the Components allow for HUSLAB to build NGS pipelines fulfilling the accreditation requirements of SFS-EN ISO 15189:2012.
Please see paragraph II.2.4 for detailed description and Annex 1.1.1 for description of the steps of the current HUSLAB WES laboratory process and The Special Requirements for High Sensitivity Variant Detection. Annex 1.1.1 applies to both lots 1 and 2.
Näytä lisää Tietoa eristä
Tarjouksia voidaan jättää kaikista eristä
1️⃣ Hankinnan laajuus
Otsikko: NGS Library Adapters ("Adapters")
Otsikko
Erän tunnistenumero: 1
Kuvaus
Lisätuotteet/palvelut: Laboratorioreagenssit📦
Suorituspaikka: Suomi/Finland🏙️
Hankinnan kuvaus:
“The following components are requested for the HUSLAB WES process using Illumina Novaseq 6000 sequencer:
- Adapters for Novaseq 6000 compatible paired-end...”
Hankinnan kuvaus
The following components are requested for the HUSLAB WES process using Illumina Novaseq 6000 sequencer:
- Adapters for Novaseq 6000 compatible paired-end libraries containing unique dual indexes (UDI) for at least 384 different samples, also carrying unique molecular identifiers (UMI). These Adapters must be incorporated before any denaturation or amplification steps resulting in an unamplified UDI + UMI barcoded library. The Adapters must contain all barcode information needed so that final library amplification is done using universal Illumina-type primers.
The requirements for the procurement are described in the paragraph "Procurement object criteria".
Please see also Annex 1.1.1.
Näytä lisää Myöntämisperusteet
Hinta ei ole ainoa myöntämisperuste, ja kaikki perusteet ilmoitetaan ainoastaan hankinta-asiakirjoissa
Sopimuksen, puitesopimuksen tai dynaamisen hankintajärjestelmän kesto
Jäljempänä oleva aikataulu on ilmaistu kuukausina.
Kuvaus
Kesto: 24
Sopimuksen, puitesopimuksen tai dynaamisen hankintajärjestelmän kesto
Tämä sopimus voidaan uusia ✅ Kuvaus
Uudistusten kuvaus:
“The Contract is in force for a Fixed Period of two (2) years, and thereafter until terminated.”
2️⃣ Hankinnan laajuus
Otsikko: Hydralization Probes ("Probes")
Otsikko
Erän tunnistenumero: 2
Kuvaus
Suorituspaikka: Manner-Suomi🏙️
Hankinnan kuvaus:
“The following components are requested for the HUSLAB WES process using Illumina Novaseq 6000 sequencer:
- Biotinylated Hybridization Probes capturing both...”
Hankinnan kuvaus
The following components are requested for the HUSLAB WES process using Illumina Novaseq 6000 sequencer:
- Biotinylated Hybridization Probes capturing both template strands. The Probes must be customizable to genomic regions of interest of HUSLAB (HUSLAB RoI) provided in hg38 reference coordinates. The size of current HUSLAB RoI is 57 Mb.
The requirements for the procurement are described in the paragraph "Procurement object criteria".
Please see also Annex 1.1.1.
Oikeudelliset, taloudelliset, rahoitukselliset ja tekniset tiedot Osallistumisehdot
Luettelo ja lyhyt kuvaus olosuhteista: Selection criteria as stated in the procurement documents.
Taloudellinen ja rahoituksellinen asema
Hankinta-asiakirjoissa mainitut valintaperusteet
Tekninen ja ammatillinen pätevyys
Hankinta-asiakirjoissa mainitut valintaperusteet
Menettely Toimenpiteen tyyppi
Avoin menettely
Hallinnolliset tiedot
Tarjousten tai osallistumishakemusten vastaanottamisen määräaika: 2022-04-08
13:00 📅
Kielet, joilla tarjoukset tai osallistumishakemukset voidaan jättää: englanti 🗣️
Jäljempänä oleva aikataulu on ilmaistu kuukausina.
Vähimmäisaika, jonka kuluessa tarjoajan on pidettävä tarjous voimassa: 6
Tarjousten avaamista koskevat ehdot: 2022-04-08
13:01 📅
Tarjousten avaamista koskevat ehdot (Tiedot valtuutetuista henkilöistä ja avaamismenettelystä):
“The times given in the call for tenders are given in Finnish time. The date of the opening of tenders is a preliminary date. The opening of tenders is not public.”
Tarjousten avaamista koskevat ehdot (Tiedot valtuutetuista henkilöistä ja avaamismenettelystä)
The times given in the call for tenders are given in Finnish time. The date of the opening of tenders is a preliminary date. The opening of tenders is not public.
Täydentävät tiedot Tietoa sähköisistä työnkuluista
Sähköistä tilaamista käytetään
Sähköinen laskutus hyväksytään
Sähköistä maksua käytetään
Arvostelurunko
Nimi: Markkinaoikeus
Postiosoite: Sörnäistenkatu 1
Postitoimipaikka: Helsinki
Postinumero: 00580
Maa: Suomi 🇫🇮
Puhelin: +358 295643300📞
Sähköposti: markkinaoikeus@oikeus.fi📧
URL: http://www.oikeus.fi/markkinaoikeus🌏
Lähde: OJS 2022/S 045-114338 (2022-03-01)
Ilmoitus tehdystä sopimuksesta (2022-11-08) Hankintaviranomainen Nimi ja osoitteet
Postitoimipaikka: Vantaa
Sähköposti: kilpailutus.laboratorio@hus.fi📧
Kohde Hankinnan laajuus
Lyhyt kuvaus:
“This public tender process was for NGS Library Adapters (also "Adapters") and Hybridization Probes (also "Probes") used in the diagnostic whole exome...”
Lyhyt kuvaus
This public tender process was for NGS Library Adapters (also "Adapters") and Hybridization Probes (also "Probes") used in the diagnostic whole exome sequencing (WES) NGS pipelines of HUSLAB, Laboratory of Genetics. The pipeline is used for preparing high quality sample libraries for Illumina NovaSeq 6000 sequencer. The HUSLAB WES workflow is based on a target enrichment step using hybridization probes to enrich DNA fragments originating from the genomic Regions of Interest ("RoI"). HUSLAB WES is used for high sensitivity variant detection of both germline and somatic DNA variations.
The Contracting Entity estimates that annual orders will be made for 2000 samples (estimation for 2022 and HUS estimates, that the number of samples is rising) divided in three to four (3-4) different separate orders.
The tender is devided into two lots:
1) NGS Library Adapters
2) Hybridization Probes
The procurement entity selects one supplier for both lots so that the lot may have either the same or a different supplier.
INTENDED USE
The Adapters and the Probes offered will be Components of the HUSLAB WES process. HUSLAB will select the Components and combine them in order to create the final HUSLAB WES process used for generation of high quality and high sensitivity customized WES sample libraries, fulfilling the Special Requirements for High Sensitivity Variant Detection.
The WES pipelines using the Adapters and the Probes are used for production of high quality WES sample libraries from DNA isolated from:
- whole blood
- freshly isolated or frozen tissue samples or cells
- FFPE samples (optionally omitting DNA fragmentation)
- cell-free DNA (omitting input DNA fragmentation)
The NGS pipelines using the Components allow for HUSLAB to build NGS pipelines fulfilling the accreditation requirements of SFS-EN ISO 15189:2012.
Näytä lisää
Hankinnan kokonaisarvo (ilman arvonlisäveroa): EUR 617 600 💰
Tietoa eristä
Tämä sopimus on jaettu osiin ✅ Kuvaus
Hankinnan kuvaus:
“The following components are requested for the HUSLAB WES process using Illumina Novaseq 6000 sequencer:
- Adapters for Novaseq 6000 compatible paired-end...”
Hankinnan kuvaus
The following components are requested for the HUSLAB WES process using Illumina Novaseq 6000 sequencer:
- Adapters for Novaseq 6000 compatible paired-end libraries containing unique dual indexes (UDI) for at least 384 different samples, also carrying unique molecular identifiers (UMI). These Adapters must be incorporated before any denaturation or amplification steps resulting in an unamplified UDI + UMI barcoded library. The Adapters must contain all barcode information needed so that final library amplification is done using universal Illumina-type primers.
Näytä lisää Myöntämisperusteet
Hinta
Kuvaus
Hankinnan kuvaus:
“The following components are requested for the HUSLAB WES process using Illumina Novaseq 6000 sequencer:
- Biotinylated Hybridization Probes capturing both...”
Hankinnan kuvaus
The following components are requested for the HUSLAB WES process using Illumina Novaseq 6000 sequencer:
- Biotinylated Hybridization Probes capturing both template strands. The Probes must be customizable to genomic regions of interest of HUSLAB (HUSLAB RoI) provided in hg38 reference coordinates. The size of current HUSLAB RoI is 57 Mb.