Electrical and Asymmetrical Flow Field Flow Fractionation (EAF4) Instrument with Online Multidetection System

Lyhyt kuvaus

Asymmetrical flow field-flow fractionator with electrical charge-based separation capacity (EAF4), light scattering detectors (MALS and DLS), concentration detectors (dRI and UV), autosampler for automated sample injection (optional), manual injection valve, automated fraction collector, analytical EAF4 — compatible channel, semi-preparative channel (optional), pumps enabling the splitting of the channel flow (optional), pump allowing automatic switching between four eluents, centrifugal FFF module (optional), computer, monitor and keyboard and the software needed for data analysis and stimulation of experiments, fluorescence detector (optional) and various channel options: frit-inlet, mini/short and micro channels (optional). In addition, trade-in discount for return of the old Postnova AF4 instrument (optional) including the AF000 MF multiflow system, degasser, channel oven, fraction collector, UV detector with analytical and preparative flow cells, analytical channel, autosampler, manual injection valve, automated fraction collector, slot flow pump.

Lyhyt kuvaus

Asymmetrical flow field-flow fractionator with electrical charge-based separation capacity (EAF4), light scattering detectors (MALS and DLS), concentration detectors (dRI and UV), autosampler for automated sample injection (optional), manual injection valve, automated fraction collector, analytical EAF4 -compatible channel, semi-preparative channel (optional), pumps enabling the splitting of the channel flow (optional), pump allowing automatic switching between four eluents, centrifugal FFF module (optional), computer, monitor and keyboard, and the software needed for data analysis and stimulation of experiments, fluorescence detector (optional) and various channel options: frit-inlet, mini/short, and micro channels (optional). In addition, trade-in discount for return of the old Postnova AF4 instrument (optional) including the AF000 MF multiflow system, degasser, channel oven, fraction collector, UV detector with analytical and preparative flow cells, analytical channel, autosampler, manual injection valve, automated fraction collector, slot flow pump.

Hankinnan kuvaus

Electrical and Asymmetrical Flow Field Flow Fractionation (EAF4) instrument with multidetection platform will be acquired for the Biomolecular complex purification unit (Biocomplex) at the University of Helsinki. Biocomplex is part of the national integrated structural biology consortium Structural Biology in Finland (FINStruct, previously Instruct-FI), structural biology unit of Helsinki Institute of Life Sciences (Instruct-HiLIFE), and pan-European research infrastructure in structural biology (Instruct-ERIC). The unit is specialized in the purification of large macromolecular complexes that meet the quality and quantity requirements for samples intended for determination of high-resolution structures as well as other types of functional and biochemical tests. The instrument will be used with aqueous solutions for analysis and purification of biological samples such as proteins and their complexes, protein aggregates, biopolymers (nucleic acids and polysaccharides), lipid vesicles, extracellular vesicles, protein-nucleic acid complexes, virus-like particles, viruses, nanoparticles, etc. Coupling to high quality online detectors is aiming at the determination of the molar mass, size, and size distribution of the studied samples. EAF4 enables obtaining information on the particle/molecule net charge and zeta potential.

Vaihtoehtojen kuvaus

Instrument procurement includes optional modules and items.
• automated sample injection with sample ampoule racks (autosampler; category A);
• controller for sample dilution in the channel (category A);
• semipreparative channel and a set of spacers (category A);
• frit inlet channel and a set of spacers (Category B);
• mini (short ~14 cm long) channel and a set of spacers (Category B);
• micro channel (~7 cm long) and a set of spacers (Category B);
• centrifugal FFF module (Category B);
• fluorescence detector (Category B);
• trade in discounts for the old instrument and modules (divided as parts 1 and 2) (category A)
Buyer asks to give the trade-in discount prices separately for the following module combinations:
1) Old Postnova AF2000 MT multiflow system; temperature controlled channel oven; Temperature controlled fraction collector; UV detector with analytical and preparative flow cells; Analytical channel; degasser;
2) Autosampler, semipreparative channel, smart stream splitter
• also as an option, category A: the estimated annual service cost (service contract) for one on-site preventive maintenance visit including consumables and travel costs.
Please, specify the content of preventive maintenance and consumables in the tender. The price is taken into account in the evaluation (Weight for the price maximum score: 40.00 %).
The price and quality of the optional modules and trade-in discounts in category A and optional annual service contract are taken into account in the evaluation of the maximal points for price and quality and thus in the sum of total points.
The price of the optional items in category B is not taken into account in the evaluation of maximal points for price.
The buyer reserves the right to include or not to include the optional items of Category A or B in the final purchase.